Place your bets for the bird sperm grand prix – all in the cause of conservation

Helen Taylor explains why she devotes her research to bird semen, and how an avian sperm race can help save one of New Zealand’s forgotten taonga, the hihi.

When I was little, I wanted to be David Attenborough, but here I am at 35 hanging out in the bush collecting bird semen samples and encouraging people to bet on which bird has the fastest sperm. I feel like I owe everyone (including my younger self) an explanation.

An interesting career choice

My mum doesn’t like to tell people what I do. She generally states that I work in bird conservation and allows me to remain a mysterious avian Jane Goodall figure in people’s minds. What my mum refuses to tell people is that, after several years of studying and a hard-earned PhD, my current research involves sampling sperm from male birds and checking it out under a microscope to see how fast it’s swimming. And I love it.

Now, as well as analysing bird sperm for conservation science, I and some colleagues are using bird sperm as a mechanic to raise money for one of New Zealand’s forgotten taonga, the hihi (or stitchbird). But, before we get to that, when I talk to people about my research, there are several questions that I understandably, routinely get asked. So let’s cover those off first.

Hihi like to feed on nectar. When there aren’t enough plants around, they rely on sugar water in special feeders. Many hihi chicks are currently hatched in nest boxes. Hihi sperm at x400 magnification. (Images: Mhairi McCready, Vix Franks, Mhairi McCready, Helen Taylor)

Why do I study bird sperm?

My work involves figuring out what happens to the genetics of populations when they get very small. Typically this leads to low genetic diversity and increased mating between relatives (inbreeding). Currently, my research specifically focusses on how inbreeding might be affecting male fertility in New Zealand birds. We know that, in groups like mammals, insects, and plants, inbreeding causes males to fire blanks. But no-one has really investigated whether the same is true for birds.

If inbreeding does lead to dodgy sperm, it could be bad news for New Zealand’s birds, many of which have experienced drastic reductions in population size thanks to introduced mammals and habitat destruction. So, as part of my Marsden-funded research programme, I visit remote islands and predator-free sanctuaries with my specially designed mobile sperm lab and check up on the sperm quality of, in particular, South Island robins and hihi (stitchbirds).

The hihi is a great example of a New Zealand bird that’s experienced a dramatic population decline. It’s not surprising that people are not familiar with this bird; hihi are really difficult to see. They were once widespread across the North Island but, thanks to the arrival of both humans and their associated problems, by 1880, hihi could only be found on Hauturu (Little Barrier Island). There are now seven populations of hihi, but they’re extremely conservation-reliant (they need nest boxes and supplementary feeding with sugar water at most sites), and we know they have been affected by inbreeding in the past. So it’s important to know what’s going on with male fertility in this species.

How do you get sperm from birds?

Ah, the classic question that usually gets asked within minutes of telling people what I do. It’s actually pretty simple. Male birds in the majority of species do not have a penis. Instead, both males and females have a cloaca – a single opening for both reproduction and excreting waste. Fun fact: cloaca comes from the Latin for sewer and Cloacina was the roman goddess of the sewage system.

Anyhow, in male passerine birds, the area round the cloaca becomes quite swollen during mating season. This swelling acts as a storage area for semen prior to mating. When we catch male birds in mating season, we can use a technique called cloacal massage to cause a small amount of semen to pool on the surface of the cloaca and then collect it.

The lucky males Helen currently works with: South Island robins and hihi. Far right: cloacal massage in action (Images: Helen Taylor, Mhairi McCready, Robyn White)

What do you measure to check the sperm quality? 

We look at two main things to judge whether a bird sperm sample is good quality or not: swimming speed and morphology. Swimming speed is just that – how fast do the sperm swim around? Morphology refers to the length of the sperm and each of its component parts (the head, the midpiece, and the tail) and also what proportion of the sperm are abnormal (two heads, missing tails etc). It’s generally agreed that faster sperm are better, and that longer sperm are faster (although there’s still a bit of debate about that).

What we can’t look at is sperm count – how many sperm in the sample. That’s because the samples we get through cloacal massage are not a true ejaculate. It’s not an accurate representation of how many sperm we would see allocated to a real ejaculate, so this would be an unfair measure.

What do you need to measure sperm quality in the wild?

Quite a lot of heavy, bulky equipment, as everyone who’d helped me lug my gear around remote, challenging locations will testify. We can measure sperm morphology back at the lab in Dunedin. Swimming speed, however, has to be measured there and then, right after collection or the sperm will die. And dead sperm are notoriously poor swimmers. There are several challenges here. We need gear to track the sperm. We need to keep the sperm at constant temperature or they’ll die. And we need electricity to run all our gear on.

To solve these issues, I designed a mobile sperm lab that I can take pretty much anywhere. It consists of a tent containing a microscope with a camera on top, connected to a laptop that runs sperm tracking software. To keep the semen warm, we have a slide warmer on the microscope, a simple, but effective, Tupperware box with a reptile heatpad inside and, the pièce de résistance, a specially designed in-bra sperm tube holder that I wear to keep samples warm against my skin. All the equipment runs off a small petrol generator that we can take with us wherever we go.

The highly sophisticated mobile sperm lab; unloading our gear in “challenging” circumstances; the incredible in-bra sperm tube organiser (seen here, out of bra) (Images: Helen Taylor, Steph Price)

Do the birds enjoy it?

Almost certainly not. But we try and make it as stress-free as possible for them and keep handling time as low as possible.

What’s all this about racing sperm?

During the 2017/18 hihi breeding season sampling sperm from male hihi across four of the seven current populations. One night, myself and two other hihi researchers, Alex Knight and Mhairi McCready, were sitting around on Tiritiri Matangi shooting the breeze about hihi and, obviously, their sperm. Light-hearted banter about running a hihi sperm sweepstake between us eventually turned into The Great Hihi Sperm Race.

The idea is simple. I have measured sperm swimming speed in 128 male hihi. All of these hihi are now listed on our website where you can place a $10 bet on which male you think will have the fastest sperm. You can bet on as many birds as you like, and whoever picks the birds with the fastest swimmers are in to win a whole bunch of prizes.

www.hihispermrace.co.nz

So, there you have it – everything you wanted to know (or didn’t even know you wanted to know) about bird sperm research and sperm-based conservation fundraising. If you have any other questions about what I do, please post them in the comments box on the site. Sorry, Mum, the cat is well and truly out of the bag now.


The Spinoff’s science content is made possible thanks to the support of The MacDiarmid Institute for Advanced Materials and Nanotechnology, a national institute devoted to scientific research.

Related:


The Spinoff is made possible by the generous support of the following organisations.
Please help us by supporting them.